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EXPRESSION OF ABCG2 TRANSPORTER DURING TRACHEAL REGENERATION IN RATS
Author(s): 
Pages: 163-167
Year: Issue:  2
Journal: ACTA ANATOMICA SINICA

Keyword:  ABCG2气管干细胞氟脲嘧啶间接免疫荧光法免疫印迹法大鼠;
Abstract: 目的观察离体大鼠气管上皮损伤修复过程中气管干细胞的动态变化.方法用氟脲嘧啶(5-FU)造成离体大鼠气管上皮损伤,应用间接免疫荧光法和Western blotting动态观测了修复过程中各时间点气管上皮ABCG2的表达.结果 1.5-FU作用12 h后大部分气管上皮脱落,残留的G0期细胞中部分可见ABCG2表达阳性.去除5-FU后3 h细胞数目增多,为扁平状,ABCG2阳性细胞数也随之增多;6~9 h上皮细胞由扁平变为立方,ABCG2阳性细胞数继续增多;24 h上皮细胞呈立方状,并连接成片,ABCG2阳性细胞较前明显减少;48 h气管上皮接近恢复假复层结构,此时仅见极少量ABCG2阳性细胞.正常气管上皮未检测到ABCG2表达.2.Western blotting分析表明,ABCG2表达量的变化趋势与免疫荧光结果一致.结论 ABCG2的表达与干细胞的数量呈正相关,可作为气管干细胞的标志物.
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