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EXPRESSION OF Wnt-1 IN RAT TRACHEAL STEM CELL DURING THE EARLY PROLIFERATION AND DIFFERENTIATION
Author(s): 
Pages: 168-171
Year: Issue:  2
Journal: ACTA ANATOMICA SINICA

Keyword:  Wnt-1气管干细胞增殖分化免疫印迹法大鼠;
Abstract: 目的检测Wnt-l在大鼠气管上皮损伤修复中的表达及其变化,探讨影响气管干细胞早期增殖分化的分子调控机制.方法氟脲嘧啶(5-FU)作用大鼠离体气管,去除5-FU后分别于0、6、12、24及48 h用Westernblotting及免疫组织化学方法检测气管上皮中Wnt-1的表达.结果 5-FU损伤后12 h,气管上皮脱落,仅残留少量G0期细胞表达Wnt-1;去除5-FU后6 h,上皮细胞数目增多,呈扁平状间断分布,Wnt-1表达最强;12 h后,上皮细胞呈立方状,并连接成片,Wnt-1表达逐渐减少;恢复48 h,气管上皮内出现假复层结构,仅见极少量Wnt-1阳性细胞.正常气管上皮Wnt-1表达极少. 结论 Wnt-1时间依赖性表达与气管上皮损伤修复进程相吻合,说明其在气管干细胞早期增殖分化中起重要调控作用.
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