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Cytohistological Observation in the Tissue Cultrue of Isatica Indicago
Author(s): 
Pages: 17-20
Year: Issue:  2
Journal: JOURNAL OF TANGSHAN TEACHERS COLLEGE

Keyword:  菘蓝外植体愈伤组织分化细胞组织学;
Abstract: 以菘蓝试管苗幼叶为外植体,MS+6-BA 0.5mg·L-1+NAA0.1 mg·L-1为愈伤组织培养基,分化培养基为MS+6-BA 2mg·L-1+NAA 0.2 mg·L-1.观察黄色正常愈伤组织和绿色水浸状愈伤组织在组织细胞学上的特性及由菘蓝愈伤组织分化不定芽和不定根的过程,得出:由菘蓝愈伤组织上分化的不定芽起源于愈伤组织的表层细胞,叶原基与原体-原套同时发生.不定芽的起源为外起源.不定根起源于维管束外薄壁细胞处,根原基向外需穿透皮层,突出愈伤组织表面,才能达到肉眼可见的程度.不定根的起源为内起源.
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