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Construction and identification of recombinant plasmid pcDNA3-β amyloid
Author(s): 
Pages: 18-19,32
Year: Issue:  1
Journal: ANATOMY RESEARCH

Keyword:  β-淀粉样蛋白DNA重组序列测定Tg2576转基因鼠;
Abstract: 目的构建β-淀粉样蛋白真核表达质粒,为进一步开展老年性痴呆DNA疫苗的保护性研究打下基础.方法提取Tg2576转基因鼠基因组DNA,PCR扩增β-淀粉样蛋白(Aβ1-42)基因,用限制性内切酶Kpn Ⅰ/Xho Ⅰ分别对扩增产物和真核表达质粒pcDNA3.1酶切,将目的基因定向克隆到pcDNA3.1载体上;对重组质粒进行双酶切初步鉴定后进行序列测定.结果特异扩增出Aβ1-42片段,大小为126 bp,片段成功插入pcDNA3.1载体中.经双酶切及序列测定结果表明Aβ1-42目的基因正确重组入pcDNA3.1载体中.结论成功构建Aβ1-42真核表达质粒.
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