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Cloning and Sequencing of Cucumis Melo Fruit-specific Expression Cucumisin Gene Promoter Region
Author(s): 
Pages: 46-49
Year: Issue:  1
Journal: ACTA SCIENTIARUM NATURALIUM UNIVERSITATIS NEIMONGOL

Keyword:  甜瓜果实特异性启动子黄瓜素;
Abstract: 黄瓜素(cucumisin)是甜瓜类枯草杆菌丝氨酸蛋白酶,其表达具有果实特异性.应用PCR方法从甜瓜基因组DNA中扩增出该基因自转录起始位点至上游一段长310 bp片段,并将其克隆到pUC19载体中,序列分析表明该序列与已报道的相应序列完全相同,且具有TATA-box、CAAT-box、G-box 、I-box-like和增强子元件TGTCACA等功能域,具有典型的果实特异性启动子特征.成功获得甜瓜果实特异性表达基因的启动子,为下一步实现外源基因在甜瓜果实中特异表达奠定基础.
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