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STUDY ON GETTING PARAMETERS OF CHLOROPHYLL FLUORESCENCE DYNAMICS BY NON-MODULATED FLUOROMETER PLANT EFFICIENCY ANALYSER
Author(s): 
Pages: 34-38
Year: Issue:  1
Journal: ACTA BIOPHYSICA SINICA

Keyword:  非调制式荧光仪调制式荧光仪叶绿素荧光动力学;
Abstract: 为了利用非调制式荧光仪获得调制式荧光仪测定的叶绿素荧光动力学参数,采用植物效率(PEA)仪进行了叶绿素荧光参数测定程式的4要素(光化光强度及其照射时间、饱和激发光强度及其照射时间)以及节约测定时间和仪器内存的测定技术研究.结果表明,暗适应10min后,在设定180 μmol·m-2·s-1光化光照射360 s时间内,以连续2次1950μmol·m-2·s-1饱和激发光照射3 s的测定程式,在7种植物的叶绿素荧光参数的测定中获得了较为满意的测定结果,而且与Strasser等测定程式相比,1次测定需时从600s缩短为360 s、耗用仪器内存从76.9%减少为2.56%.
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