Effect of adrenomedullin on tissue factors and TFPI expression in HUVECs treated with oxLDL
Author(s): LIU Wei, ZHU Zi-qiang, ZU Shu-yu, ZHU Guang-jin
Pages: 12-16
Year: 2006 Issue: 1
Journal: CHINESE JOURNAL OF PATHOPHYSIOLOGY
Keyword: 肾上腺髓质素; 脂蛋白; LDL; 动脉硬化; 凝血致活酶;
Abstract: 目的:探讨肾上腺髓质素(AM)对氧化低密度脂蛋白(oxLDL)影响脐静脉内皮细胞(HUVECs)组织因子(TF)及其抑制物(TFPI)表达的干预作用,并探讨可能的信号转导途径.方法:进行人HUVECs原代培养,分别采用发色底物法和RT-PCR技术检测TF及TFPI蛋白活性及mRNA水平的变化,并给予8-溴-环腺苷酸(cAMP拮抗剂)、PD098059(MAPKK抑制剂)及H7(PKC抑制剂)观察AM影响TFPI表达的信号途径.结果:AM呈剂量依赖性地抑制oxLDL诱导的HUVECs TF蛋白活性和mRNA表达的升高;AM可增加HUVECs TFPI的表达,并能减轻oxLDL所致的TFPI蛋白活性和mRNA水平的降低;AM通过cAMP和MAPK途径影响TFPI的变化.结论:AM可以减低oxLDL对HUVECs TF和TFPI表达的影响,进而改善动脉粥样硬化的凝血状态,延缓其进程.
Pages: 12-16
Year: 2006 Issue: 1
Journal: CHINESE JOURNAL OF PATHOPHYSIOLOGY
Keyword: 肾上腺髓质素; 脂蛋白; LDL; 动脉硬化; 凝血致活酶;
Abstract: 目的:探讨肾上腺髓质素(AM)对氧化低密度脂蛋白(oxLDL)影响脐静脉内皮细胞(HUVECs)组织因子(TF)及其抑制物(TFPI)表达的干预作用,并探讨可能的信号转导途径.方法:进行人HUVECs原代培养,分别采用发色底物法和RT-PCR技术检测TF及TFPI蛋白活性及mRNA水平的变化,并给予8-溴-环腺苷酸(cAMP拮抗剂)、PD098059(MAPKK抑制剂)及H7(PKC抑制剂)观察AM影响TFPI表达的信号途径.结果:AM呈剂量依赖性地抑制oxLDL诱导的HUVECs TF蛋白活性和mRNA表达的升高;AM可增加HUVECs TFPI的表达,并能减轻oxLDL所致的TFPI蛋白活性和mRNA水平的降低;AM通过cAMP和MAPK途径影响TFPI的变化.结论:AM可以减低oxLDL对HUVECs TF和TFPI表达的影响,进而改善动脉粥样硬化的凝血状态,延缓其进程.
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